Planting the seeds for a forest of RNAi pathways

Cells from most eukaryotic species make several different types of small interfering RNAs.Pioneering work in plants, published in PLOS Biology almost 20 years ago, established a framework to Theatre Box’s understand how multiple RNA interference pathways can regulate the genome in parallel.This Perspective looks back at a classic PLOS Biology paper that provided an early glimpse of the complexity Strings of RNAi pathways in plants and how it acted as a springboard for further exploration of this extravagant universe.